Rat liver lysosomes contain an arylamidase which has molecular weight of 135,000, and is separated into seven forms under isoelectric focusing. One form of these arylamidases, having isoelectric point of 7.8(form IV), was purified from rat liver by conventional chromatographic techniques. Final preparation of form VI appeared homogeneous by polyacrylamide disk electrophoresis and consisted of three identical polypeptide chain, as judged by disk electrophoresis in the presence of sodium dodecyl sulfate. The enzyme hydrolyzed naphthylamides of neutral amino acids but did not hydrolyze naphthy...
