Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Jun Watanabe
Tomoyuki Oki
Jun Takebayashi
Koji Yamasaki
Yuko Takano-Ishikawa
Akihiro Hino
Akemi Yasui

We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

TAYLOR & FRANCIS LTD

10.1271/bbb.120786

https://doi.org/10.1271/bbb.120786
https://www.ncbi.nlm.nih.gov/pubmed/23563538
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000319095500030&DestApp=WOS_CPL