論文

基本情報

氏名 沖 智之
氏名(カナ) オキ トモユキ
氏名(英語) OKI TOMOYUKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

DMAC法による豆類中の総プロアントシアニジンの定量法

単著・共著の別

 

著者

沖智之, 菅原晃美, 古川(佐藤)麻紀, 須田郁夫

概要

A method based on the 4-dimethylaminocinnamaldehyde (DMAC) method was optimized for determination of total proanthocyanidin content in grain legumes using 96-well microplates. The recovery determined for a milled soybean material spiked with black soybean seed coat extract equivalent to 1.0, 2.0 and 4.2 mg/g of proanthocyanidin ranged from 96.1 to 97.9%. Duplicate determinations of total proanthocyanidin content in eight different grain legumes were performed on three different days. Statistical treatment revealed that the relative standard deviation of the repeatability (RSDr) and the relative standard deviation of the intermediate reproducibility (RSDint) for determination of total proanthocyanidin content was 0.5 to 4.1% and 0.9 to 4.7 %, respectively. The HorRat values (RSDint/predicted RSDR) ranged from 0.17 to 0.87. This demonstrates that the proposed method for determining total proanthocyanidin content in grain legumes had good intralaboratory reproducibility. The contents of proanthocyanidin, ranging from 0.33 to 2.97 mg/g, in 59 samples of black soybean determined by the DMAC method were strongly correlated to those determined by the vanillin-sulfuric acid method (r=0.980

発表雑誌等の名称

日本食品科学工学会誌

出版者

日本食品科学工学会

60

6

開始ページ

301

終了ページ

309

発行又は発表の年月

20130615

査読の有無

無し

招待の有無

無し

記述言語

日本語

掲載区分

 

掲載種別

 

ISSN

1341-027X

ID:DOI

10.3136/nskkk.60.301

ID:NAID(CiNiiのID)

10031177110

ID:PMID

 

URL

JGlobalID

201302258701802138

arXiv ID

 

ORCIDのPut Code

 

DBLP ID

 

OpenDepoのWekoID