論文

基本情報

氏名 中野 裕史
氏名(カナ) ナカノ ヒロシ
氏名(英語) HIROSHI NAKANO
所属 中村学園大学 教育学部 児童幼児教育学科
職名 教授

題名

Short-term adenosine monophosphate-activated protein kinase activator 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside treatment increases the sirtuin 1 protein expression in skeletal muscle.

単著・共著の別

 

著者

Suwa M
Nakano H
Radak Z
Kumagai S

担当区分

 

概要

Adenosine monophosphate-activated protein kinase (AMPK) has been proposed to stimulate mitochondrial biogenesis and fat and glucose metabolism in skeletal muscle. Nicotinamide adenine dinucleotide-dependent histone deacetylase sirtuin 1 (SIRT1) is also thought to play a pivotal role for such metabolic adaptations. The purpose of the present study was to examine the effect of AMPK activation with the administration of AMPK activator 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) to rats on skeletal muscle SIRT1 protein expression as well as peroxisome proliferator activated receptor gamma coactivator-1 alpha (PGC-1 alpha) and glucose transporter 4 (GLUT4) protein expression and hexokinase activity. The AICAR promoted the phosphorylation of AMPK alpha-subunit (Thr(172)) and acetyl-coenzyme A carboxylase (Ser(79)) without any change of total AMPK alpha-subunit or acetyl-coenzyme A carboxylase protein levels in both the slow-twitch soleus and fast-twitch extensor digitorum longus (EDL) muscles. The SIRT1 protein expression increased at 24 hours after administration of AICAR in the EDL muscle but not in the soleus muscle. The PGC-1 alpha protein expression increased in both the soleus and EDL muscles and GLUT4 did in the EDL muscle at 24 hours after an administration of AICAR. The hexokinase activity increased at 18 and 24 hours in the soleus and at 12, 18, and 24 hours in the EDL after an AICAR treatment. These results suggest that short-term AICAR treatment to rats promotes skeletal muscle AMPK phosphorylation and then coincidently increases the SIRT1 protein expression. In addition, such treatment also enhances the PGC-1 alpha and GLUT4 protein contents and hexokinase activity in skeletal muscle. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.

発表雑誌等の名称

Metabolism: clinical and experimental

出版者

3

60

3

開始ページ

394

終了ページ

403

発行又は発表の年月

2011-03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1016/j.metabol.2010.03.003

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID