Morphological and histochemical changes of tissue structures in persimmon leaves were investigated using a fresh leaf, a freeze - dried powder and a semi-purified dietary fiber preparation isolated from the powder. Concentration and composition of dietary fiber (DF) and other chemical components in the latter two samples were also analyzed. Morphological observation showed that the fresh persimmon leaf distributed palisade and spongy parenchyma between the upper and lower epidermis. It was observed by histochemical techniques that the cell walls and vacuoles of the cells were stained strong...