論文

基本情報

氏名 山本 健太
氏名(カナ) ヤマモト ケンタ
氏名(英語) YAMAMOTO KENTA
所属 中村学園大学 栄養科学部 フード・マネジメント学科
職名 講師

題名

Utilization of Single Nucleotide Polymorphism-based Allele-specific PCR to Identify Shiikuwasha (Citrus depressa Hayata) and Calamondin (Citrus madurensis Lour.) in Processed Juice

単著・共著の別

 

著者

Musou-Yahada, Ayumi
Honjoh, Ken-ichi
Yamamoto, Kenta
Miyamoto, Takahisa
Ohta, Hideaki

担当区分

 

概要

To develop a method for the identification of shiikuwasha (Citrus depressa Hayata) and calamondin (Citrus madurensis Lour.), trnL-trnF and trnT-trnL intergenic spacer regions of their chloroplast DNA were amplified using PCR and the nucleotide sequences were determined. In each region, a single nucleotide polymorphism (SNP) site specific to the respective citrus species (shiikuwasha and calamondin) was found. For species discrimination using PCR, two forward primers containing the allele-specific SNP site at the 3'-end and a mismatched nucleotide at the 3rd base from the 3'-end were designed. The allele-specific forward primers specific to shiikuwasha and calamondin were respectively designated CiDeLF-F and CiMaTL-F. To confirm the specificity of the designed primers, PCR was carried out with DNA prepared from citrus peel or hand-squeezed juice as the template. Results showed that shiikuwasha and calamondin fruits and juices were identifiable by PCR using the allele-specific primers. Furthermore, this allele-specific PCR method can be applied to industrially processed and concentrated juice by amplifying DNA in advance.

発表雑誌等の名称

FOOD SCIENCE AND TECHNOLOGY RESEARCH

出版者

JAPANESE SOC FOOD SCI & TECHNOLOGY

25

1

開始ページ

19

終了ページ

27

発行又は発表の年月

2019-01

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.3136/fstr.25.19

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID