論文

基本情報

氏名 信久 幾夫
氏名(カナ) ノブヒサ イクオ
氏名(英語) NOBUHISA IKUO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters

単著・共著の別

 

著者

Ikuo Nobuhisa
Mitsujiro Osawa
Mami Uemura
Yoko Kishikawa
Maha Anani
Kaho Harada
Haruna Takagi
Kiyoka Saito
Masami Kanai-Azuma
Yoshiakira Kanai
Atsushi Iwama
Tetsuya Tagaa

担当区分

 

概要

During mouse development, definitive hematopoiesis is first detected around embryonic day 10.5 (E10.5) in the aorta-gonad-mesonephros (AGM) region, which exhibits intra-aortic cell clusters. These clusters are known to contain hematopoietic stem cells (HSCs). On the other hand, it is not clear how the cells in such clusters maintain their HSC phenotype and how they are triggered to differentiate. Here we found that an endodermal transcription factor marker, Sox17, and other F-group (SoxF) proteins, Sox7 and Sox18, were expressed in E10.5 intra-aortic cell clusters. Forced expression of any of these SoxF proteins, particularly Sox17, in E10.5AGMCD45low c-Kithigh cells, which are the major component of intra-aortic clusters, led to consistent formation of cell clusters in vitro during several passages of cocultures with stromal cells. Cluster-forming cells with constitutive Sox17 expression retained long-term bone marrow reconstitution activity in vivo. Notably, shutdown of exogenously introduced Sox17 gene expression resulted in immediate hematopoietic differentiation. These results indicate that SoxF proteins, especially Sox17, contribute to the maintenance of cell clusters containing HSCs in the midgestationAGMregion. Furthermore, SoxF proteins play a pivotal role in controlling the HSC fate decision between indefinite self-renewal and differentiation during fetal hematopoiesis. © 2014, American Society for Microbiology.

発表雑誌等の名称

Molecular and Cellular Biology

出版者

American Society for Microbiology

34

11

開始ページ

1976

終了ページ

1990

発行又は発表の年月

2014

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1128/MCB.01485-13

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID