論文

基本情報

氏名 信久 幾夫
氏名(カナ) ノブヒサ イクオ
氏名(英語) NOBUHISA IKUO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Identification of the B Subtype of gamma Phospholipase A(2 9 Inhibitor from Protobothrops flavoviridis Serum and Molecular Evolution of Snake Serum Phospholipase A(2) Inhibitors.

単著・共著の別

 

著者

So S
Chijiwa T
Ikeda N
Nobuhisa I
Oda-Ueda N
Hattori S
Ohno M

担当区分

 

概要

A cDNA encoding a novel phospholipase A(2) (PLA(2)) inhibitor (PLI) was isolated from a Protobothrops flavoviridis snake (Tokunoshima island, Japan) liver cDNA library. This cDNA encoded a signal peptide of 19 amino acids followed by a mature protein of 181 amino acids. Its N-terminal amino acid sequence was completely in accord with that of a PLI, named PLI-II, previously found in P. flavoviridis serum. PLI-II showed a high similarity in sequence to the B subtype of gamma PLI, denoted gamma PLI-B, isolated from Agkistrodon blomhoffii siniticus serum. Thus, PLI-II is P. flavoviridis serum gamma PLI-B. Since PLI-I, previously isolated from P. flavoviridis serum, can be assigned as gamma PLI-A, P. flavoviridis serum contains both A and B subtypes of gamma PLI. Phylogenetic analysis of gamma PLIs from the sera of various kinds of snakes, Elapinae, Colubrinae, Laticaudinae, Acanthophiinae, Crotalinae, and Pythonidae, based on the amino acid sequences revealed that A and B subtypes of gamma PLIs are clearly separated from each other. It was also found that phylogenetic topologies of gamma PLIs are in good agreement with speciation processes of snakes. The BLAST search followed by analyses with particular Internet search engines of proteins with Cys/loop frameworks similar to those of PLI-II and PLI-I revealed that gamma PLI-Bs, including PLI-II and PLI-II-like proteins from mammalian sources, form a novel PLI-II family which possesses the common Cys/loop frameworks in the anterior and posterior three-finger motifs in the molecules. Several lines of evidence suggest that PLI-II is evolutionarily ancestral to PLI-I.

発表雑誌等の名称

J Mol Evol

出版者

SPRINGER

66

3

開始ページ

298

終了ページ

307

発行又は発表の年月

2008-03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1007/s00239-008-9089-1

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID