論文

基本情報

氏名 藤原 秀彦
氏名(カナ) フジハラ ヒデヒコ
氏名(英語) FUJIHARA HIDEHIKO
所属 中村学園大学 栄養科学部 フード・マネジメント学科
職名 准教授

題名

A New ICEclc Subfamily Integrative and Conjugative Element Responsible for Horizontal Transfer of Biphenyl and Salicylic Acid Catabolic Pathway in the PCB-Degrading Strain Pseudomonas stutzeri KF716.

単著・共著の別

 

著者

Jun Hirose
Takahito Watanabe
Taiki Futagami
Hidehiko Fujihara
Nobutada Kimura
Hikaru Suenaga
Masatoshi Goto
Akiko Suyama
Kensuke Furukawa

担当区分

 

概要

Integrative and conjugative elements (ICEs) are chromosomally integrated self-transmissible mobile genetic elements. Although some ICEs are known to carry genes for the degradation of aromatic compounds, information on their genetic features is limited. We identified a new member of the ICEclc family carrying biphenyl catabolic bph genes and salicylic acid catabolic sal genes from the PCB-degrading strain Pseudomonas stutzeri KF716. The 117-kb ICEbph-salKF716 contains common core regions exhibiting homology with those of degradative ICEclc from P. knackmussii B13 and ICEXTD from Azoarcus sp. CIB. A comparison of the gene loci collected from the public database revealed that several putative ICEs from P. putida B6-2, P, alcaliphila JAB1, P. stutzeri AN10, and P. stutzeri 2A20 had highly conserved core regions with those of ICEbph-salKF716, along with the variable region that encodes the catabolic genes for biphenyl, naphthalene, toluene, or phenol. These data indicate that this type of ICE subfamily is ubiquitously distributed within aromatic compound-degrading bacteria. ICEbph-salKF716 was transferred from P. stutzeri KF716 to P. aeruginosa PAO1 via a circular extrachromosomal intermediate form. In this study, we describe the structure and genetic features of ICEbph-salKF716 compared to other catabolic ICEs.

発表雑誌等の名称

Microorganisms

出版者

 

9

12

開始ページ

 

終了ページ

 

発行又は発表の年月

2021-11-29

査読の有無

有り

招待の有無

 

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

国際誌

国際共著

 

ISSN

 

eISSN

 

DOI

10.3390/microorganisms9122462

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID