Efficient screening of environmental isolates for Saccharomyces cerevisiae strains that are suitable for brewing.

Hidehiko Fujihara
Mika Hino
Hideharu Takashita
Yasuhiro Kajiwara
Keiko Okamoto
Kensuke Furukawa

We developed an efficient screening method for Saccharomyces cerevisiae strains from environmental isolates. MultiPlex PCR was performed targeting four brewing S. cerevisiae genes (SSU1, AWA1, BIO6, and FLO1). At least three genes among the four were amplified from all S. cerevisiae strains. The use of this method allowed us to successfully obtain S. cerevisiae strains.

Bioscience, biotechnology, and biochemistry

TAYLOR & FRANCIS LTD

10.1080/09168451.2014.910098

https://doi.org/10.1080/09168451.2014.910098
https://www.ncbi.nlm.nih.gov/pubmed/25036140
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