MISC

基本情報

氏名 藤原 秀彦
氏名(カナ) フジハラ ヒデヒコ
氏名(英語) FUJIHARA HIDEHIKO
所属 中村学園大学 栄養科学部 フード・マネジメント学科
職名 准教授

題名

Characterization of the second LysR-type regulator in the biphenyl-catabolic gene cluster of Pseudomonas pseudoalcaligenes KF707

単著・共著の別

 

著者

T Watanabe
H Fujihara
K Furukawa

担当区分

 

概要

Pseudomonas pseudoalcaligenes KF707 possesses a biphenyl-catabolic (bph) gene cluster consisting of bphR1A1A2-(orf3)-bphA3A4BCX0X1X2X3D. The bphR1 (formerly orf0) gene product, which belongs to the GntR family, is a positive regulator for itself and bphX0X1X2X3D. Further analysis in this study revealed that a second regulator belonging to the LysR family (designated bphR2) is involved in the regulation of the bph genes in KF707. The bphR2 gene was not located near the bph gene cluster, and its product (BphR2) exhibited a high level of similarity to NahR (the naphthalene- and salicylate-catabolic regulator belonging to the LysR family) in plasmid NAH7 of Pseudomonas putida. A strain containing a disrupted bphR2 gene failed to grow on biphenyl as a sole source of carbon, and the BphD (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase) activity was significantly reduced compared to that of wild-type strain KF707. Furthermore, the same strain exhibited extremely low transcription of bphR1, bphA1, bphC, bphX0, and bphD. However, when the bphR2 gene was provided in trans to the bphR2-disrupted strain, the transcription level of these genes was restored. These results indicate that bphR2 regulates the bph genes positively as a second regulator together with BphR1.

発表雑誌等の名称

JOURNAL OF BACTERIOLOGY

出版者

AMER SOC MICROBIOLOGY

185

12

開始ページ

3575

終了ページ

3582

発行又は発表の年月

2003-06

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1128/JB.185.12.3575-3582.2003

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID