論文

基本情報

氏名 安武 健一郎
氏名(カナ) ヤスタケ ケンイチロウ
氏名(英語) YASUTAKE KENICHIRO
所属 中村学園大学 栄養科学部 栄養科学科
職名 准教授

題名

Expression profile of lipid metabolism-associated genes in hepatitis C virus-infected human liver

単著・共著の別

 

著者

Tatsuya Fujino
Makoto Nakamuta
Ryoko Yada
Yoko Aoyagi
Kenichiro Yasutake
Motoyuki Kohjima
Kunitaka Fukuizumi
Tsuyoshi Yoshimoto
Naohiko Harada
Masayoshi Yada
Masaki Kato
Kazuhiro Kotoh
Akinobu Taketomi
Yoshihiko Maehara
Manabu Nakashima
Munechika Enjoji

担当区分

 

概要

Aim:
Recent studies have shown that lipid metabolic pathways are required for the entry, replication and secretion of hepatitis C virus (HCV). Although little is known about the life cycle of HCV in humans, the activation of cholesterol and fatty acid biosynthesis may be critical for HCV proliferation.
Methods:
We assessed the transcription levels of genes essential for cholesterol and fatty acid biosynthesis in liver samples obtained from patients with chronic hepatitis C and determined their correlations. The serum levels of low-density lipoprotein (LDL) cholesterol and HCV core antigen were also measured.
Results:
The gene expression of the LDL receptor (LDLR) was suppressed, whereas that of SREBP1c, liver X receptor-alpha (LXR alpha), fatty acid synthase (FASN), and HMG-CoA reductase and synthase (HMGR and HMGS) was significantly increased, and SREBP2 transcription was comparable in HCV-infected liver compared with normal liver. Positive correlations were found for LDLR versus HMGR, HMGR versus SREBP1c, and LDLR versus SREBP2 in the HCV-infected and control liver. Although the LXR alpha-SREBP1c-FASN pathway was upregulated, proteasome activator 28 gamma (PA28 gamma) was downregulated at the transcriptional level in HCV-infected liver, and was not significantly correlated with the other genes examined. The serum LDL cholesterol level was negatively correlated with LDLR and HMGR expression.
Conclusion:
These results suggest that, in HCV-infected liver, the cholesterol load increases and cholesterol uptake is controlled, while de novo cholesterol synthesis is upregulated compared with the normal physiological state. The positive correlations in the expression levels of some cholesterol metabolism-associated genes indicate that not all of the metabolic pathways are dysregulated in HCV-infected liver.

発表雑誌等の名称

HEPATOLOGY RESEARCH

出版者

WILEY-BLACKWELL

40

9

開始ページ

923

終了ページ

929

発行又は発表の年月

2010-09

査読の有無

無し

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1111/j.1872-034X.2010.00700.x

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID