論文

基本情報

氏名 安武 健一郎
氏名(カナ) ヤスタケ ケンイチロウ
氏名(英語) YASUTAKE KENICHIRO
所属 中村学園大学 栄養科学部 栄養科学科
職名 准教授

題名

Expression Profiles of Genes Associated With Viral Entry in HCV-Infected Human Liver

単著・共著の別

 

著者

M. Nakamuta
T. Fujino
R. Yada
Y. Aoyagi
K. Yasutake
M. Kohjima
K. Fukuizumi
T. Yoshimoto
N. Harada
M. Yada
M. Kato
K. Kotoh
A. Taketomi
Y. Maehara
M. Nakashima
M. Enjoji

担当区分

 

概要

Recent studies have demonstrated that several cellular factors are involved in entry of hepatitis C virus (HCV) into host cells. Detailed gene expression profiles of these factors in HCV-infected livers have not been reported for humans. Transcriptional levels of LDL receptor (LDLR), CD81, scavenger receptor class B type I (SR-BI), claudin-1, and occludin genes in liver samples from patients with chronic hepatitis C were investigated. Serum levels of LDL-cholesterol (LDL-C) and HCV core antigen were also evaluated, and expression of claudin-1 and occludin were immunohistochemically analyzed. Compared with normal liver, transcription of LDLR and claudin-1 genes was significantly suppressed (P < 0.0001) and occludin transcription was significantly up-regulated in HCV-infected livers (P < 0.0001). Significant positive correlations were found for LDLR versus occludin, LDLR versus claudin-1, occludin versus claudin-1, and CD81 versus SR-BI in HCV-infected (P = 0.0012, P < 0.0001, P = 0.0004, and P < 0.0001, respectively) and normal livers (P < 0.0001, P = 0.0051, P < 0.0001, and P < 0.0001, respectively). Positive correlation was observed between serum levels of HCV core antigen and LDL-C (P = 0.0147), with their levels negatively correlated to LDLR (P - 0.0270 and P - 0.0021, respectively). Immunohistochemically, hepatocellular expression of claudin-1 and occludin was increased in HCV-infected livers. Different levels of expression were demonstrated at the mRNA and protein levels for occludin and claudin-1 in HCV-infected and normal livers. Correlation of elements associated with viral entry was comparable in HCV-infected and normal livers. J. Med. Virol. 83: 921-927, 2011. (C) 2011 Wiley-Liss, Inc.

発表雑誌等の名称

JOURNAL OF MEDICAL VIROLOGY

出版者

WILEY-BLACKWELL

83

5

開始ページ

921

終了ページ

927

発行又は発表の年月

2011-05

査読の有無

無し

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1002/jmv.22042

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID