論文

基本情報

氏名 竹嶋 美夏子
氏名(カナ) タケシマ ミカコ
氏名(英語) MIKAKO TAKESHIMA
所属 中村学園大学 栄養科学部 フード・マネジメント学科
職名 准教授

題名

Anti-proliferative and apoptosis-inducing activity of lycopene against three subtypes of human breast cancer cell lines

単著・共著の別

 

著者

Mikako Takeshima
Misaki Ono
Takako Higuchi
Chen Chen
Takayuki Hara
Shuji Nakano

担当区分

 

概要

Although lycopene, a major carotenoid component of tomatoes, has been suggested to attenuate the risk of breast cancer, the underlying preventive mechanism remains to be determined. Moreover, it is not known whether there are any differences in lycopene activity among different subtypes of human breast cancer cells. Using ER/PR positive MCF-7, HER2-positive SK-BR-3 and triple-negative MDA-MB-468 cell lines, we investigated the cellular and molecular mechanism of the anticancer activity of lycopene. Lycopene treatment for 168 consecutive hours exhibited a time-dependent and dose-dependent anti-proliferative activity against these cell lines by arresting the cell cycle at the G(0)/G(1) phase at physiologically achievable concentrations found in human plasma. The greatest growth inhibition was observed in MDA-MB-468 where the sub-G(0)/G(1) apoptotic population was significantly increased, with demonstrable cleavage of PARP. Lycopene induced strong and sustained activation of the ERK1/2, with concomitant cyclin D1 suppression and p21 upregulation in these three cell lines. In triple negative cells, lycopene inhibited the phosphorylation of Akt and its downstream molecule mTOR, followed by subsequent upregulation of proapoptotic Bax without affecting anti-apoptotic Bcl-xL. Taken together, these data indicate that the predominant anticancer activity of lycopene in MDA-MB-468 cells suggests a potential role of lycopene for the prevention of triple negative breast cancer.
The anticancer effects of lycopene vary with subtypes of breast cancer, with the greatest inhibition observed in triple negative cells where the anticancer activity could be brought not only by an arrest of cell cycle progression at G0/G1 phase, but also by apoptosis induction. Our findings provide evidence explaining preferential preventive activity of lycopene against ER-negative breast cancer including triple negative breast cancer reported in epidemiological studies.

発表雑誌等の名称

CANCER SCIENCE

出版者

WILEY-BLACKWELL

105

3

開始ページ

252

終了ページ

257

発行又は発表の年月

2014-03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1111/cas.12349

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID