論文

基本情報

氏名 竹嶋 美夏子
氏名(カナ) タケシマ ミカコ
氏名(英語) MIKAKO TAKESHIMA
所属 中村学園大学 栄養科学部 フード・マネジメント学科
職名 准教授

題名

Differential anti-tumor activities of curcumin against Ras- and Src-activated human adenocarcinoma cells

単著・共著の別

 

著者

Misaki Ono
Takako Higuchi
Mikako Takeshima
Chen Chen
Shuji Nakano

担当区分

 

概要

Although curcumin has been studied as a potential anticancer drug targeting multiple signaling molecules, the role of oncogenic Src and Ras in curcumin sensitivity remains unknown. Using HAG-1 human adenocarcinoma cells transfected with either activated Src or Ras, we investigated here the functional role of these oncogenes in curcumin sensitivity. Activation of either Src or Ras did not confer resistance to curcumin, compared to vehicle-transfected cells. Curcumin enhanced Erk1/2 predominantly in Ras-activated cells, but inhibited Akt and its downstream molecules (mTOR and S6K1) regardless of these oncogene activations. The sub-G0/G1 apoptotic populations were substantially increased with demonstrable cleavage of PARP, but this increase was most prominent in Src-activated cells. Suppression of Bcl-xL level and enhanced expression of Bax were demonstrated in Src-activated, but not Ras-activated cells. By contrast, drastic increases of G(2)/M cell populations were seen in Ras-activated cells rather than Src-activated cells, suggesting a potential role of Ras/Erk1/2 activation in curcumin-induced G(2)/M arrest These data indicate that curcumin-induced growth inhibition would be mediated mainly by G(2)/M arrest in Ras-driven cells but by apoptosis induction in Src-driven cells, providing a mechanistic rationale for the potential use of curcumin in the treatment of human cancers with activated Src or Ras. (C) 2013 Elsevier Inc. All rights reserved.

発表雑誌等の名称

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

出版者

ACADEMIC PRESS INC ELSEVIER SCIENCE

436

2

開始ページ

186

終了ページ

191

発行又は発表の年月

2013-06

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1016/j.bbrc.2013.05.071

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID