論文

基本情報

氏名 末武 勲
氏名(カナ) スエタケ イサオ
氏名(英語) SUETAKE ISAO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

マウスDnmt1 DNAメチルトランスフェラーゼによるヘミメチル化CpG部位のプロセス的メチル化

単著・共著の別

 

著者

VILKAITIS G
SUETAKE I
KLIMASAUSKAS S
TAJIMA S

担当区分

 

概要

DNA methyltransferase Dnmt1 ensures clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during replication. Dnmt1 is targeted to replication foci, interacts with PCNA, and favors methylating the hemimethylated form of CpG sites. To understand the underlying mechanism of its maintenance function, we purified recombinant forms of full-length Dnmt1, a truncated form of Dnmt1-(291-1620)lacking the binding sites for PCNA and DNA and examined their processivity using a series of long unmethylated and hemimethylated DNA substrates. Direct analysis of methylation patterns using bisulfite-sequencing and hairpin-PCR techniques demonstrated that full-length Dnmt1 methylates hemimethylated DNA with high processivity and a fidelity of over 95%, but unmethylated DNA with much less processivity. The truncated form of Dnmt1 showed identical properties to full-length Dnmt1 indicating that the N-terminal 290-amino acid residue region of Dnmt1 is not required for preferential activity toward hemimethylated sites or for processivity of the enzyme. Remarkably, our analyses also revealed that Dnmt1 methylates hemimethylated CpG sites on one strand of double-stranded DNA during a single processive run. Our findings suggest that these inherent enzymatic properties of Dnmt1 play an essential role in the faithful and efficient maintenance of methylation patterns in the mammalian genome.

発表雑誌等の名称

Journal of Biological Chemistry

出版者

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

280

1

開始ページ

64

終了ページ

72

発行又は発表の年月

2005

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1074/jbc.M411126200

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID