論文

基本情報

氏名 末武 勲
氏名(カナ) スエタケ イサオ
氏名(英語) SUETAKE ISAO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

naked及びヌクレオソームDNAに対するDnmt3a及びDnmt3bの個別のDNAメチル化活性

単著・共著の別

 

著者

TAKESHIMA Hideyuki
SUETAKE Isao
SHIMAHARA Hideto
URA Kiyoe
TATE Shin-ichi
TAJIMA Shoji

担当区分

 

概要

In mammals, the resetting of DNA methylation patterns in early embryos and germ cells is crucial for development. De novo type DNA methyltransferases Dnmt3a and Dnmt3b are responsible for creating DNA methylation patterns during embryogenesis and in germ cells. Although their in vitro DNA methylation properties are similar, Dnmt3a and Dnmt3b methylate different genomic DNA regions in vivo. In the present study, we have examined the DNA methylation activity of Dnmt3a and Dnmt3b towards nucleo-somes reconstituted from recombinant histones and DNAs, and compared it to that of the corresponding naked DNAs. Dnmt3a showed higher DNA methylation activity than Dnmt3b towards naked DNA and the naked part of nucleosomal. DNA. On the other hand, Dnmt3a scarcely methylated the DNA within the nucleosome core region, while Dnmt3b significantly did, although the activity was low. We propose that the preferential DNA methylation activity of Dnmt3a towards the naked part of nucleosomal DNA and the significant methylation activity of Dnmt3b towards the nucleosome core region contribute to their distinct methylation of genomic DNA in vivo.

発表雑誌等の名称

Journal of Biochemistry

出版者

JAPANESE BIOCHEMICAL SOC

139

3

開始ページ

503

終了ページ

515

発行又は発表の年月

2006

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1093/jb/mvj044

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID