論文

基本情報

氏名 末武 勲
氏名(カナ) スエタケ イサオ
氏名(英語) SUETAKE ISAO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Array-based genomic resequencing of human leukemia

単著・共著の別

 

著者

Y. Yamashita
J. Yuan
I. Suetake
H. Suzuki
Y. Ishikawa
Y. L. Choi
T. Ueno
M. Soda
T. Hamada
H. Haruta
S. Takada
Y. Miyazaki
H. Kiyoi
E. Ito
T. Naoe
M. Tomonaga
M. Toyota
S. Tajima
A. Iwama
H. Mano

担当区分

 

概要

To identify oncogenes in leukemias, we performed large-scale resequencing of the leukemia genome using DNA sequence arrays that determine similar to 9 Mbp of sequence corresponding to the exons or exon-intron boundaries of 5648 protein-coding genes. Hybridization of genomic DNA from CD34-positive blasts of acute myeloid leukemia (n = 19) or myeloproliferative disorder (n = 1) with the arrays identified 9148 nonsynonymous nucleotide changes. Subsequent analysis showed that most of these changes were also present in the genomic DNA of the paired controls, with 11 somatic changes identified only in the leukemic blasts. One of these latter changes results in a Met-to-Ile substitution at amino-acid position 511 of Janus kinase 3 (JAK3), and the JAK3(M511I) protein exhibited transforming potential both in vitro and in vivo. Further screening for JAK3 mutations showed novel and known transforming changes in a total of 9 out of 286 cases of leukemia. Our experiments also showed a somatic change responsible for an Arg-to-His substitution at amino-acid position 882 of DNA methyltransferase 3A, which resulted in a loss of DNA methylation activity of >50%. Our data have thus shown a unique profile of gene mutations in human leukemia. Oncogene (2010) 29, 3723-3731; doi: 10.1038/onc.2010.117; published online 19 April 2010

発表雑誌等の名称

ONCOGENE

出版者

NATURE PUBLISHING GROUP

29

25

開始ページ

3723

終了ページ

3731

発行又は発表の年月

2010-06

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1038/onc.2010.117

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID