論文

基本情報

氏名 末武 勲
氏名(カナ) スエタケ イサオ
氏名(英語) SUETAKE ISAO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

HP1αのヒンジとクロモシャドウはヌクレオソームにおいてK9メチル化ヒストンH3の認識に関与する

単著・共著の別

 

著者

MISHIMA Yuichi
WATANABE Makoto
KAWAKAMI Toru
JAYASINGHE Chanika D.
OTANI Junji
KIKUGAWA Yusuke
SHIRAKAWA Masahiro
KIMURA Hiroshi
NISHIMURA Osamu
AIMOTO Saburo
TAJIMA Shoji
SUETAKE Isao

担当区分

 

概要

The majority of the genome in eukaryotes is packaged into transcriptionally inactive chromatin. Heterochromatin protein 1 (HP1) is a major player in the establishment and maintenance of heterochromatin. HP1 specifically recognizes a methylated lysine residue at position 9 in histone H3 through its N-terminal chromo domain (CD). To elucidate the binding properties of HP1 alpha to nucleosomes in vitro, we reconstituted nudeosomes containing histone H3 trimethylated at lysine 9. HP1 alpha exhibited high-affinity binding to nucleosomes containing methylated histone H3 in a nucleosome core-number-dependent manner. The hinge region (HR) connecting the CD and C-terminal chromoshadow domain (CSD), and the CSD contributed to the selective binding of HP1 alpha to histone H3 with trimethylated lysine 9 through weak DNA binding and by suppressing the DNA binding, respectively. We propose that not only the specific recognition of lysine 9 methylation of histone H3 by the CD but also the HR and the CSD cooperatively contribute to the selective binding of HP1 a to histone H3 lysine 9 methylated nucleosomes. (C) 2012 Elsevier Ltd. All rights reserved.

発表雑誌等の名称

Journal of Molecular Biology

出版者

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

425

1

開始ページ

54

終了ページ

70

発行又は発表の年月

2013

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1016/j.jmb.2012.10.018

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID