論文

基本情報

氏名 末武 勲
氏名(カナ) スエタケ イサオ
氏名(英語) SUETAKE ISAO
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

ミセルに閉じ込めた酸化剤を使用した触媒酸化亜硫酸水素塩基配列決定による5-ヒドロキシメチルシトシンの単純かつ正確な単一塩基解像度解析

単著・共著の別

 

著者

Fukuzawa Seketsu
Fukuzawa Seketsu
Takahashi Saori
Tachibana Kazuo
Tajima Shoji
Suetake Isao
Suetake Isao

担当区分

 

概要

Oxidation of 5-methylcytosine (5mC) is catalyzed by ten-eleven translocation (TET) enzymes to produce 5-hydroxymethylcytosine (5hmC) and following oxidative products. The oxidized nucleotides were shown to be the intermediates for DNA demethylation, as the nucleotides are removed by base excision repair system initiated by thymine DNA glycosylase. A simple and accurate method to determine initial oxidation product 5hmC at single base resolution in genomic DNA is necessary to understand demethylation mechanism. Recently, we have developed a new catalytic oxidation reaction using micelle-incarcerated oxidants to oxidize 5hmC to form 5-formylcytosine (5fC), and subsequent bisulfite sequencing can determine the positions of 5hmC in DNA. In the present study, we described the optimization of the catalytic oxidative bisulfite sequencing (coBS-seq), and its application to the analysis of 5hmC in genomic DNA at single base resolution in a quantitative manner. As the oxidation step showed quite low damage on genomic DNA, the method allows us to down scale the sample to be analyzed. (C) 2016 Elsevier Ltd. All rights reserved.

発表雑誌等の名称

Bioorganic & Medicinal Chemistry

出版者

PERGAMON-ELSEVIER SCIENCE LTD

24

18

開始ページ

4254

終了ページ

4262

発行又は発表の年月

2016

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1016/j.bmc.2016.07.016

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID