Very recently, we have reported 2, 2', 3, 4', 5, 5', 6-heptachlorobiphenyl (heptaCB) (CB187)could be metabolized to two monohydroxy (0H)-metabolites and one dechlorinated OH metabolite by liver microsomes of rats, hamsters and guinea pigs. In this study, in order to determine the metabolic pathways of CB187, we tried to synthesize two OH-PCBs, namely4'-OH-2, 2', 3, 3', 5, 5', 6-heptaCB (CB178) and 3'-OH-CB187. After the diazo-coupling reaction with2, 3, 5, 6-tetrachloroaniline and 2, 3, 6-trichlorophenol as starting materials, the reaction mixture was applied to Silica gel 60 column chromat...
