論文

基本情報

氏名 太田 千穂
氏名(カナ) オオタ チホ
氏名(英語) CHIHO OHTA
所属 中村学園大学 栄養科学部 栄養科学科
職名 准教授

題名

Effect of quercetin on the uptake and efflux of aristolochic acid I from Caco-2 cell monolayers

単著・共著の別

 

著者

Osamu Kimura
Yukiko Fujii
Koichi Haraguchi
Chiho Ohta
Nobuyuki Koga
Yoshihisa Kato
Tetsuya Endo

担当区分

 

概要

ObjectiveThe purpose of this study was to determine whether quercetin decreases the uptake of aristolochic acid I (AAI) from the apical membranes of Caco-2 cells via H+-linked MCTs at neutral pH as well as to confirm the secretion of AAI through the Caco-2 cell monolayers via ABC transporters.
MethodsCaco-2 cells cultured on the dishes or permeable membranes were incubated with AAI in the absence or presence of quercetin or transporter inhibitors.
Key findingsCoincubation with quercetin decreased the uptake of AAI by Caco-2 cells cultured on the dishes at pH 7.4, and a similar decrease in AAI uptake was found when the cells were coincubated with acetic acid or benzoic acid. In contrast, the basolateral-to-apical transport of AAI was higher than the apical-to-basolateral transport of AAI at pH 7.4, and the former transport was decreased by quercetin and the BCRP inhibitors of Ko-143 and mitoxantrone, but not by P-gp or MRP2 inhibitors.
ConclusionsAAI appears to be secreted from the apical membranes of Caco-2 cells via BCRP at neutral pH, although a small amount of AAI is taken up from the apical membranes via H+-linked MCTs, and quercetin may decrease both the BCRP-mediated efflux and the MCT-mediated influx of AAI.

発表雑誌等の名称

JOURNAL OF PHARMACY AND PHARMACOLOGY

出版者

WILEY-BLACKWELL

68

7

開始ページ

883

終了ページ

889

発行又は発表の年月

2016-07

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1111/jphp.12557

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID