論文

基本情報

氏名 太田 千穂
氏名(カナ) オオタ チホ
氏名(英語) CHIHO OHTA
所属 中村学園大学 栄養科学部 栄養科学科
職名 准教授

題名

Uptake of perfluorooctanoic acid by Caco-2 cells: Involvement of organic anion transporting polypeptides

単著・共著の別

 

著者

Osamu Kimura
Yukiko Fujii
Koichi Haraguchi
Yoshihisa Kato
Chiho Ohta
Nobuyuki Koga
Tetsuya Endo

担当区分

 

概要

The mechanism underlying the intestinal absorption of perfluorooctanoic acid (PFOA) was investigated using Caco-2 cells. The uptake of PFOA from the apical membrane of Caco-2 cells was fast, and pH, temperature, and concentration dependent, but Na+ independent. Coincubation with sulfobromophthalein (BSP), glibenclamide, estron-3-sulfate, cyclosporine A or rifamycin SV, which are typical substrates or inhibitors of organic anion transporting polypeptides (OATPs), significantly decreased the uptake of PFOA. However, coincubation with probenecid or p-aminohippuric acid, typical substrates of organic anion transporters, did not decrease the uptake of PFOA. Furthermore, coincubation with L-lactic acid or benzoic acid, substrates of monocarboxylic acid transporters, did not decrease PFOA uptake. The relationship between the initial uptake of PFOA and its concentration was saturable, suggesting the involvement of a carrier-mediated process. The calculated K-m and uptake clearance (V-max/K-m) values for PFOA were 8.3 mu M and 55.0 mu L/mg protein/min, respectively. This clearance value was about 3-fold greater than that of the non-saturable uptake clearance (K-d: 18.1 mu L/mg protein/min). Lineweaver-Burk plots revealed that BSP competitively inhibits the uptake of PFOA, with a K-i value of 23.1 mu M. These results suggest that the uptake of PFOA from the apical membranes of Caco-2 cells could be, at least in part, mediated by OATPs along with BSP.

発表雑誌等の名称

TOXICOLOGY LETTERS

出版者

ELSEVIER IRELAND LTD

277

 

開始ページ

18

終了ページ

23

発行又は発表の年月

2017-08

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1016/j.toxlet.2017.05.012

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID