論文

基本情報

氏名 加藤 正樹
氏名(カナ) カトウ マサキ
氏名(英語) KATO MASAKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

The effects of unsaturated fatty acids on lipid metabolism in HepG2 cells

単著・共著の別

著者

Motoyuki Kohjima
Munechika Enjoji
Nobito Higuchi
Masaki Kato
Kazuhiro Kotoh
Manabu Nakashima
Makoto Nakamuta

担当区分

概要

We investigated the effects of stearic acid (saturated), oleic acid (monounsaturated), linoleic acid (n-6 polyunsaturated), and alpha-linolenic acid (n-3 polyunsaturated) on lipid metabolism in a hepatocyte-derived cell line, HepG2. HepG2 cells were cultured in medium supplemented with either stearic acid (0.1% w/v), oleic acid (0.1% v/v), linoleic acid (0.1% v/v), or alpha-linolenic acid (0.1% v/v). After 24 h, expression of lipid metabolism-associated genes was evaluated by real-time PCR. alpha-Linolenic acid showed a suppressive effect on the hepatic fatty acid de novo synthesis and fatty acid oxidation pathways, while linoleic acid also showed a tendency to suppress these pathways although the effect was weaker. Moreover, alpha-linolenic acid enhanced the expression of enzymes associated with reactive oxygen species (ROS) elimination. In contrast, oleic acid tended to promote fatty acid synthesis and oxidation. In conclusion, alpha-linolenic acid and linoleic acid may be expected to ameliorate hepatic steatosis by downregulating fatty acid de novo synthesis and fatty acid oxidation, and by upregulating ROS elimination enzymes. Oleic acid had no distinct effects for improving steatosis or oxidative stress.

発表雑誌等の名称

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

出版者

SPRINGER

45

1

開始ページ

6

終了ページ

9

発行又は発表の年月

2009/01/01

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

国際共著

ISSN

1071-2690

eISSN

DOI

10.1007/s11626-008-9144-7

Cinii Articles ID

Cinii Books ID

Pubmed ID

PubMed Central 記事ID

形式

無償ダウンロード

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID