論文

基本情報

氏名 加藤 正樹
氏名(カナ) カトウ マサキ
氏名(英語) KATO MASAKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Changes in the expression of cholesterol metabolism-associated genes in HCV-infected liver: A novel target for therapy?

単著・共著の別

著者

Makoto Nakamuta
Ryoko Yada
Tatsuya Fujino
Masayoshi Yada
Nobito Higuchi
Masatake Tanaka
Masayuki Miyazaki
Motoyuki Kohjima
Masaki Kato
Tsuyoshi Yoshimoto
Naohiko Harada
Akinobu Taketomi
Yoshihiko Maehara
Momoko Koga
Takuya Nishinakagawa
Manabu Nakashima
Kazuhiro Kotoh
Munechika Enjoji

担当区分

概要

Recent investigations indicate that hepatitis C virus (HCV) infection is closely associated with hepatocytic lipid metabolism and induces hepatic steatosis. However, the actual lipid metabolism in HCV-infected liver has not been extensively investigated in humans. In this Study, we evaluated the expression of lipid metabolism-associated genes in patients with HCV infection by real-time PCR. Sterol regulatory element-binding protein (SREBP)-2 expression was unchanged and low density lipoprotein receptor expression was markedly reduced by 90% in HCV-infected liver. The expression of apolipoprotein B100, microsomal triglyceride transfer ptotein and ATP-binding cassette G5 was significantly increased. Up-regulation of cholesterol synthesis-associated genes, including HMG-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase and squalene synthase, confirmed enhanced de novo cholesterol synthesis. The expression of cholesterol 7 alpha-hydroxylase and farnesoid X receptor was enhanced, while bile salt export pump expression was unchanged. Fatty acid synthase expression was increased which was accompanied by increased expression of liver X receptor a and SREBP-1c. In summary, the regulation of lipid metabolism was impaired and cholesterol and fatty acid synthesis continued to increase without negative feedback in HCV-infected liver. These changes may be beneficial for HCV replication.

発表雑誌等の名称

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

出版者

SPANDIDOS PUBL LTD

24

6

開始ページ

825

終了ページ

828

発行又は発表の年月

2009/12/01

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

国際共著

ISSN

1107-3756

eISSN

DOI

10.3892/ijmm_00000299

Cinii Articles ID

Cinii Books ID

Pubmed ID

PubMed Central 記事ID

形式

無償ダウンロード

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID