MISC

基本情報

氏名 住本 英樹
氏名(カナ) スミモト ヒデキ
氏名(英語) SUMIMOTO HIDEKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Anionic amphiphile-independent activation of the phagocyte NADPH oxidase in a cell-free system by p47(phox) and p67(phox), both in C terminally truncated forms - Implication for regulatory Src homology 3 domain-mediated interactions

単著・共著の別

 

著者

K Hata
T Ito
K Takeshige
H Sumimoto

担当区分

 

概要

Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b(558) comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Here we show that, even without the amphiphiles, the oxidase is activated in vitro by a C terminally truncated p47(phox)., retaining the N-terminal and the two SH3 domains, and the N terminus of p67(phox). When either truncated p47(phox) or, p67(phox) is replaced by the respective full-length one, the activation absolutely requires the amphiphiles. The results indicate that both p47(phox) and p67(phox) are the primary targets of the amphiphiles, and that their C-terminal regions play negative regulatory roles. We also find that the truncated p47(phox), but not the full-length one, can bind to p22(phox), a binding required for the oxidase activation. The N-terminal SH3 domain of p47(phox) is responsible for the binding not only to p22(phox), but also to the p47(phox) C terminus. Thus the SH3 domain is accessible in the active p47(phox) but is normally masked in the full-length one probably via intramolecularly interacting with the C terminus. The present findings support our previous proposal of regulatory SH3 domain-mediated interactions.

発表雑誌等の名称

JOURNAL OF BIOLOGICAL CHEMISTRY

出版者

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

273

7

開始ページ

4232

終了ページ

4236

発行又は発表の年月

1998-02

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1074/jbc.273.7.4232

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID