MISC

基本情報

氏名 住本 英樹
氏名(カナ) スミモト ヒデキ
氏名(英語) SUMIMOTO HIDEKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils

単著・共著の別

 

著者

T Akasaki
H Koga
H Sumimoto

担当区分

 

概要

The small GTPase Rac participates in various cellular events such as cytoskeletal reorganization. It has remained, however, largely unknown about intracellular signaling pathways for Rac activation because of the lack of a simple and reliable assay to estimate the activation. Here we describe a novel method to detect the GTP-bound, active Rac in cells by pulling it down with the Rac-binding domain of the protein kinase PAK. Experiments using this method reveal that stimulation of human neutrophils with the G(i)-coupled receptor agonists N-formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B-4 (LTB4) leads to a rapid and transient increase in the GTP-bound state of Rac2, whereas phorbol myristate acetate (PMA) causes a slow but more sustained activation of Rac2. Pretreatment of cells with pertussis toxin results in defective activation of Rac2 in response to fMLP and LTB4, indicating that coupling of the receptors to G(i) plays a crucial role in the activation. Furthermore, the phosphoinositide 3-kinase (PI3K) inhibitors wortmannin and LY294002 block Rac2 activation elicited by the receptor agonists, but not that by PMA. Thus the G(i)-coupled receptors likely mediate Rac2 activation via PI3K, whereas PMA activates Rac2 in a PI3K-independent manner.

発表雑誌等の名称

JOURNAL OF BIOLOGICAL CHEMISTRY

出版者

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

274

25

開始ページ

18055

終了ページ

18059

発行又は発表の年月

1999-06

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1074/jbc.274.25.18055

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID