MISC

基本情報

氏名 住本 英樹
氏名(カナ) スミモト ヒデキ
氏名(英語) SUMIMOTO HIDEKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Tetratricopeptide repeat (TPR) motifs of p67(phox) participate in interaction with the small GTPase Rac and activation of the phagocyte NADPH oxidase

単著・共著の別

 

著者

H Koga
H Terasawa
H Nunoi
K Takeshige
F Inagaki
H Sumimoto

担当区分

 

概要

The small GTPase Rac functions as a molecular switch in several important cellular events including cytoskeletal reorganization and activation of the phagocyte NADPH oxidase, the latter of which leads to production of superoxide, a precursor of microbicidal oxidants. During formation of the active oxidase complex at the membrane, the GTP-bound Rac appears to interact with the N-terminal region of p67(phox), another indispensable activator that translocates from the cytosol upon phagocyte stimulation. Here we show that the p67(phox) N terminus lacks the CRIB motif, a well known Rac target, but contains four tetratricopeptide repeat (TPR) motifs with highly alpha-helical structure. Disruption of any of the N-terminal three TPRs, but the last one, results in defective interaction with Rac, while all the four are required for the NADPH oxidase activation. We also find that Arg-102 in the third repeat is likely involved in binding to Rac via an ionic interaction, and that replacement of this residue with Glu completely abrogates the capability of activating the oxidase both in vivo and in vitro, Thus the TPR motifs of p67(phox) are packed to function as a Rac target, thereby playing a crucial role in the active oxidase complex formation.

発表雑誌等の名称

JOURNAL OF BIOLOGICAL CHEMISTRY

出版者

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

274

35

開始ページ

25051

終了ページ

25060

発行又は発表の年月

1999-08

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1074/jbc.274.35.25051

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID