MISC

基本情報

氏名 住本 英樹
氏名(カナ) スミモト ヒデキ
氏名(英語) SUMIMOTO HIDEKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

HIV-1 TAT ACTS AS A PROCESSIVITY FACTOR INVITRO IN CONJUNCTION WITH CELLULAR ELONGATION-FACTORS

単著・共著の別

 

著者

H KATO
H SUMIMOTO
P POGNONEC
CH CHEN
CA ROSEN
RG ROEDER

担当区分

 

概要

The HIV-1 trans-activator Tat increases the rate of transcription from the HIV-1 LTR promoter through the stem-loop-containing TAR RNA. To analyze the mechanisms of Tat action, a cell-free trans-activation system with no preincubation has been developed. Recombinant Tat specifically increased the level of a long runoff transcript but not a promoter-proximal transcript in a TAR-dependent fashion. These observations and the result of pulse-chase experiments support strongly the hypothesis that Tat enhances the ability of RNA polymerase to elongate over longer distances. Increased levels of the purified cellular factor TFIIF, essential for initiation and also implicated in elongation of transcription, obviated trans-activation by Tat by increasing the basal (Tat-independent) activity. However, another elongation factor, ATN/TFIIS, showed synergistic activation with Tat. An antiserum against a recombinant form of the large subunit of TFIIF (RAP 74) preferentially suppressed the activated level of transcription exerted by Tat. We propose the hypothesis that Tat acts as a processivity factor on RNA polymerase II in an analogous manner to TFIIF.

発表雑誌等の名称

GENES & DEVELOPMENT

出版者

COLD SPRING HARBOR LAB PRESS

6

4

開始ページ

655

終了ページ

666

発行又は発表の年月

1992-04

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1101/gad.6.4.655

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID