MISC

基本情報

氏名 住本 英樹
氏名(カナ) スミモト ヒデキ
氏名(英語) SUMIMOTO HIDEKI
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

The PC motif: a novel and evolutionarily conserved sequence involved in interaction between p40(phox) and p67(phox), SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase

単著・共著の別

 

著者

R Nakamura
H Sumimoto
K Mizuki
K Hata
T Ago
S Kitajima
K Takeshige
Y Sakaki
T Ito

担当区分

 

概要

The superoxide-generating NADPH oxidase, dormant in resting phagocytes, is activated during phagocytosis following assembly of the membrane-integrated protein cytochrome b(558) and cytosolic factors. Among the latter are the three proteins containing Src homology 3(SH3) domains,p67(phox),p47(phox) and p40(phox). While the first two factors are indispensable for the activity, p40(phox) is tightly associated with p67(phox) in resting cells and is suggested to have some modulatory role. Here we describe a systematic analysis of the interaction between p40(phox) and p67(phox) using the yeast two-hybrid system and in vitro binding assays with recombinant proteins. Both methods unequivocally showed that the minimum requirements for stable interaction are the C-terminal region of p40(phox) and the region between the two SH3 domains of p67(phox). This interaction is maintained even in the presence of anionic amphiphiles used for the activation of the NADPH oxidase, raising a possibility that it mediates constitutive association of the two factors in both resting and activated cells. The C-terminal region of p40(phox) responsible for the interaction contains a characteristic stretch of amino acids designated as the PC motif, that also exists in other signal-transducing proteins from yeast to human. Intensive site-directed mutagenesis to the motif in p40(phox) revealed that it plays a critical role in the binding to p67(phox). Thus the PC motif appears to represent a novel module for protein-protein interaction used in a variety of signaling pathways.

発表雑誌等の名称

EUROPEAN JOURNAL OF BIOCHEMISTRY

出版者

SPRINGER VERLAG

251

3

開始ページ

583

終了ページ

589

発行又は発表の年月

1998-02

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1046/j.1432-1327.1998.2510583.x

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID