論文

基本情報

氏名 河手 久弥
氏名(カナ) カワテ ヒサヤ
氏名(英語) HISAYA KAWATE
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Intracellular localization and function of DNA repair methyltransferase in human cells

単著・共著の別

共著

著者

Toru Ishibashi
Yusaku Nakabeppu
Hisaya Kawate
Kunihiko Sakumi
Hiroshi Hayakawa
Mutsuo Sekiguchi

担当区分

概要

An antibody preparation specific for human O6-methylguanine-DNA methyltransferase (EC 2.1.1.63) was obtained by immunoaffinity purification on two types of affinity columns with the purified human and mouse methyltransferase proteins as ligands. The antibodies were used in Western blotting analysis of fractionated cell extracts. More than 90% of the methyltransferase protein was recovered in the cytoplasmic fractions with both human HeLa S3 cells and MR-M cells, the latter overproducing the enzyme 36 time as much as the former. Cytoplasmic localization of the methyltransferase in HeLa S3 cells was further confirmed by in situ immunostaining. By Western blotting analysis of fractionated cell extracts from HeLa S3 cells treated with alkylating agents, we found that amounts of the enzyme decreased more rapidly in the nuclear fraction than in the cytoplasmic fraction, and recovery of the enzyme level in the cytoplasmic fraction was slower than that in the other. These results suggest that the methyltransferase protein is degraded in the nucleus after it commits the repair reaction and that the cytoplasmic enzyme is transported into the nucleus as the nuclear methyltransferase is used up in this manner. © 1994.

発表雑誌等の名称

Mutation Research-DNA Repair

出版者

315

3

開始ページ

199

終了ページ

212

発行又は発表の年月

1994

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

国際誌

国際共著

国際共著していない

ISSN

eISSN

DOI

10.1016/0921-8777(94)90032-9

Cinii Articles ID

Cinii Books ID

Pubmed ID

7526198

PubMed Central 記事ID

形式

無償ダウンロード

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID