論文

基本情報

氏名 日野 真一郎
氏名(カナ) ヒノ シンイチロウ
氏名(英語) HINO SHINICHIROU
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Estrogen regulates mitochondrial morphology through phosphorylation of dynamin-related protein 1 in MCF7 human breast cancer cells.

単著・共著の別

著者

Oo PS
Yamaguchi Y
Sawaguchi A
Kyaw MTH
Choijookhuu N
Ali MN
Srisowanna N
Hino S-I
Hishikawa Y

担当区分

概要

Estrogen affects mitochondrial function in various tissues, but the precise mechanism remains unclear. We, therefore investigated the effect on estrogen-regulated mitochondrial morphology by dynamin-related protein 1 (Drp1) and its Ser616-phosphorylated derivative (pDrp1Ser616) are involved in mitochondrial fission. MCF7 human breast cancer cells were treated with 17β-estradiol (E2), an estrogen receptor (ER) α and β antagonist (ICI 182, 780), an ERα antagonist (MPP), and an ERβ antagonist (PHTPP) for 24 hr. The expression of Drp1 and pDrp1Ser616 was analyzed by western blotting and immunohistochemistry. Mitochondrial morphology was analyzed by transmission electron microscopy (TEM). In control cells, Drp1 was detected in the cytoplasm of all cells while pDrp1 was observed in the cytoplasm of 3.4 ± 1.0% of the total population. After E2 treatment, pDrp1Ser616-positive cells comprised 30.6 ± 5.6% of the total population, 10.5 ± 1.7% after E2 + ICI treatment, 12.4 ± 4.2% after E2 + MPP treatment, and 24.0 ± 2.2% after E2 + PHTPP treatment. In ERα knockdown MCF7 cells, pDrp1 expression was decreased after E2 treatment compared to E2-treated wild type cells. Tubular pattern mitochondria were found in the control cells but the number of short and small pattern mitochondria (&lt 0.5 μm2) was significantly increased after E2 treatment (as observed by TEM). We, therefore concluded that the phosphorylation of Drp1 is important for E2-dependent mitochondrial morphological changes through ERα.

発表雑誌等の名称

Acta Histochemica et Cytochemica

出版者

Japan Society of Histochemistry and Cytochemistry

51

1

開始ページ

21

終了ページ

31

発行又は発表の年月

2018/02

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

国際共著

ISSN

eISSN

DOI

10.1267/ahc.17034

Cinii Articles ID

Cinii Books ID

Pubmed ID

PubMed Central 記事ID

形式

DOI

無償ダウンロード

無償ダウンロード不可

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID