論文

基本情報

氏名 日野 真一郎
氏名(カナ) ヒノ シンイチロウ
氏名(英語) HINO SHINICHIROU
所属 中村学園大学 栄養科学部 栄養科学科
職名 教授

題名

Autophagy is activated for cell survival after endoplasmic reticulum stress

単著・共著の別

 

著者

Maiko Ogata
Shin-ichiro Hino
Atsushi Saito
Keisuke Morikawa
Shinichi Kondo
Soshi Kanemoto
Tomohiko Murakami
Manabu Taniguchi
Ichiro Tanii
Kazuya Yoshinaga
Sadao Shiosaka
James A. Hammarback
Fumihiko Urano
Kazunori Imaizumi

担当区分

 

概要

Eukaryotic cells deal with accumulation of unfolded proteins in the endoplasmic reticulum (ER) by the unfolded protein response, involving the induction of molecular chaperones, translational attenuation, and ER-associated degradation, to prevent cell death. Here, we found that the autophagy system is activated as a novel signaling pathway in response to ER stress. Treatment of SK-N-SH neuroblastoma cells with ER stressors markedly induced the formation of autophagosomes, which were recognized at the ultrastructural level. The formation of green fluorescent protein (GFP)-LC3-labeled structures (GFP-LC3 "dots"), representing autophagosomes, was extensively induced in cells exposed to ER stress with conversion from LC3-I to LC3-II. In IRE1-deficient cells or cells treated with c-Jun N-terminal kinase (JNK) inhibitor, the autophagy induced by ER stress was inhibited, indicating that the IRE1-JNK pathway is required for autophagy activation after ER stress. In contrast, PERK-deficient cells and ATF6 knockdown cells showed that autophagy was induced after ER stress in a manner similar to the wild-type cells. Disturbance of autophagy rendered cells vulnerable to ER stress, suggesting that autophagy plays important roles in cell survival after ER stress.

発表雑誌等の名称

MOLECULAR AND CELLULAR BIOLOGY

出版者

AMER SOC MICROBIOLOGY

26

24

開始ページ

9220

終了ページ

9231

発行又は発表の年月

2006-12

査読の有無

無し

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

国際・国内誌

 

国際共著

 

ISSN

 

eISSN

 

DOI

10.1128/MCB.01453-06

Cinii Articles ID

 

Cinii Books ID

 

Pubmed ID

 

PubMed Central 記事ID

 

形式

無償ダウンロード

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID